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phage brafv600e  (Sino Biological)


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    Structured Review

    Sino Biological phage brafv600e
    Phage Brafv600e, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phage+brafv600e/BRAF+(V600E)%2C+Active/pm39392755-665-5-33
    Average 92 stars, based on 2 article reviews
    phage brafv600e - by Bioz Stars, 2026-10
    92/100 stars

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    Generated:

    Article Title: ERK1/2 interaction with DHPS regulates eIF5A deoxyhypusination independently of ERK kinase activity.
    Article Snippet: Construction of the lentiviral pHAGE-DRaf:ER, pHAGE-BRAFV600E, pHAGE-MEK1-R4F (DN3/S218E/S222D, constitutively active MEK1), pLL3.7-shERK1, and pLL3.7-shERK2 was previously described.14,57 C-terminally HA-tagged DHPS in pHAGE-GFP was generated by ligating the full length DHPS cDNA from pCMV3-ORF-HA (Sino Biologicals) to the NheI/XhoI sites in pHAGE-GFP. .. Construction of the lentiviral pHAGE-DRaf:ER, pHAGE-BRAFV600E, pHAGE-MEK1-R4F (DN3/S218E/S222D, constitutively active MEK1), pLL3.7-shERK1, and pLL3.7-shERK2 was previously described.14,57 C-terminally HA-tagged DHPS in pHAGE-GFP was generated by ligating the full length DHPS cDNA from pCMV3-ORF-HA (Sino Biologicals) to the NheI/XhoI sites in pHAGE-GFP. .. The DNA encoding full-length human deoxyhypusine synthase (Uniprot: P49366, residues 1–369) and optimized for bacterial expression was synthesized along with N-teminal 6xHis-tag followed by TEV cleavage site and cloned into pET-24d(+) vector using NcoI/BamHI restriction sites (Genescript).



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